Russian Journal of BiotherapyRussian Journal of BiotherapyРоссийский биотерапевтический журнал1726-97841726-9792Publishing House ABV Press910.17650/1726-9784-2015-14-1-43-51ORIGINAL REPORTSОРИГИНАЛЬНЫЕ СТАТЬИCOMPARATIVE IN VITRO STUDY OF DIFFERENT CLASSES OF PHOTOSENSITIZERS. PYROPHEOPHORBIDES AND CHLORINESСРАВНИТЕЛЬНОЕ ИЗУЧЕНИЕ ФОТОСЕНСИБИЛИЗАТОРОВ РАЗЛИЧНЫХ КЛАССОВ В СИСТЕМЕ IN VITRO. ПИРОФЕОФОРБИДЫ И ХЛОРИНЫYakubovskayaR. I.ЯкубовскаяР. И.
Russian Federation
PlyutinskayaA. D.ПлютинскаяАнна Дмитриевна
Russian Federation
anna2031@rambler.ruPlotnikovaE. A.ПлотниковаЕ. А.
Russian Federation
GrinM. A.ГринМ. А.
Russian Federation
MironovA. F.МироновА. Ф.
Russian Federation
P.A. Hertsen Moscow Research Institute of OncologyМосковский научно-исследовательский онкологический институт им. П.А. Герцена - Филиал ФГБУ «Федерального медицинского исследовательского центра им. П.А. Герцена» Минздрава РФLomonosov State Academy of Fine Chemical Technology«Московский государственный университет тонких химических технологий им. М.В. Ломоносова»30032015141435110042018https://bioterapevt.abvpress.ru/jour/article/view/9

The study evaluates physical, chemical and biological properties of glycosylated derivatives of pyropheophorbide-α and chlorine e6, cycloimid derivatives of chlorine p6. Glycosylated derivatives of pyropheophorbide α and chlorine e6 were characterized by stability in solution without light exposure, but subject to photobleaching during exposure. Derivatives of pyropheophorbide-α showed high photo-induced activity in biological assay, the IC50 value varied from 35±7nM to 82±7 nM (depending on the pendent group and the tumor cell culture). Among the derivatives of chlorine e6 conjugate with galactose residue in A-pyrrole showed the maximum activity towards HEp2, A549 and HT29 cells (IC50 - 15±8 nM, 70±19 nM and 34±16 nM respectively), its activity 8-10 times exceeding the unconjugated counterpart and the derivatives with different macrocycle modification. Cycloimid derivatives of chlorine p6 are stable in solution without exposure and relatively stable under light exposure. These compounds showed high photo-induced activity in biological assay (IC50 varied from 35±10 nM to 250±30 nM) significantly exceeding the activity of unmodified derivatives of chlorine p6.

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