Russian Journal of BiotherapyRussian Journal of BiotherapyРоссийский биотерапевтический журнал1726-97841726-9792Publishing House ABV Press81810.17650/1726-9784-2016-15-3-28-33ORIGINAL REPORTSОРИГИНАЛЬНЫЕ СТАТЬИACTION INTERFERONS AND IFN-INDUCTORS ON TLR/RLRS GENES EXPRESSION AND DIFFERENTIATION OF TUMOR CELL LINES THP-1 AND HCT-116ДЕЙСТВИЕ ИНТЕРФЕРОНОВ И ИНДУКТОРОВ ИНТЕРФЕРОНОВ НА ЭКСПРЕССИЮ ГЕНОВ РЕЦЕПТОРОВ TLR/RLR И ДИФФЕРЕНЦИРОВКУ ОПУХОЛЕВЫХ ЛИНИЙ КЛЕТОК ТНР-1 И НСТ-116SokolovaT. M.СоколоваТатьяна Михайловна
Russian Federation
tmsokolovavir@mail.ruPoloskovV. V.ПолосковВ. В.
Russian Federation
BurovaO. S.БуроваО. С.
Russian Federation
ShuvalovA. N.ШуваловА. Н.
Russian Federation
SokolovaZ. A.СоколоваЗ. А.
Russian Federation
InshakovA. N.ИншаковА. Н.
Russian Federation
ShishkinYu. V.ШишкинЮ. В.
Russian Federation
ErshovF. I.ЕршовФ. И.
Russian Federation
ФГБУ«ФНИЦэпидемиологии и микробиологии им. Н.Ф. Гамалеи» Минздрава РоссииФГБУ «Российский онкологический научный центр им. Н.Н. Блохина» Минздрава России30092016153283310042018https://bioterapevt.abvpress.ru/jour/article/view/818

Objective. To study of known interferons (IFN) type I and IFN-inductors drugs different chemical structure on cell differentiation and expression group of genes of TLR/RLRs, referring to group of pattern-recognition receptors. Materials and methods. Cellular lines ТНР-1 (acute monocytic leukemia) и НСТ-116 (colon adenocarcinoma) were treated by interferons drugs Reaferon, Altevir, Genfakson, Infibeta 105-106 МЕ, IFN-inductors Ridostin 102-103 ßg/ml, Cycloferon 625 ßg/ml and Imunomax 2 МЕ during 24 h or 96 h at 37 °С. Gene expression were estimated by method qRT-PCR (CFX-96, Bio-Rad). CD-immuno-phenotypes of ТНР-1 cells were detected by flow cytometric fluorescence method with FITC and PE monoclonal antibodies (FACSCanto II, Becton Dickinson). Results. It was shown that tumor cells THP-1 and HCT-116 have low and variable constitutive levels gene expression of TLR-receptors 2, 3, 4, 7, 8, 9 during passages. This gene status may be connected with incorrect processing of mature mRNA forms. Genes TLR4, TLR8 and factor NFkB are stimulated by interferons and IFN-inductors more high in THP-cells and genes TLR7, TLR8, TLR9 and NFkB - in HCT-cells. Ridostin (mix ssRNA and dsRNA S. cerevisiae) is the best activator of TLR/RLRs receptors. Ridostin increases macrophage marker CD11b, Cycloferon, Imunomax stimulate levels of early T-cell antigen CD7, interferons results in growth of HLA DR. The drugs Cycloferon, Imunomax and Reaferon decrease levels of myeloid suppressor CD38. Conclusion. Group of recombinant IFNs and IFN-inductor drugs various chemical structures regulate TLR/RLRs genes expression differently in THP-1 and HCT-116. TLR/RLRs genes activation are accompanied by CD-phenotypes changes in THP-1 monocytic cell line.

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