Russian Journal of BiotherapyRussian Journal of BiotherapyРоссийский биотерапевтический журнал1726-97841726-9792Publishing House ABV Press81510.17650/1726-9784-2016-15-3-06-14ORIGINAL REPORTSОРИГИНАЛЬНЫЕ СТАТЬИE-CADHERIN EXPRESSION DOWNREGULATION ELEVATES TUMOROGENIC POTENTIAL OF HUMAN COLON CANCER CELL LINE HCT116 VIA INCREASE IN CANCER STEM CELLS AMOUNTПовышение туморогенного потенциала клеток рака ободочной кишки человека линии НСТ116 за счет увеличения содержания раковых стволовых клеток при подавлении экспрессии E-кадгеринаFarmakovskayaM. D.ФармаковскаяМ. Д.
Russian Federation
KhromovaN. V.ХромоваН. В.
Russian Federation
KopninB. P.КопнинБ. П.
Russian Federation
KopninP. B.КопнинПавел Борисович
Russian Federation
pbkopnin@mail.ruФГБУ «Российский онкологический научный центр им. Н.Н. Блохина» Минздрава России3009201615361410042018https://bioterapevt.abvpress.ru/jour/article/view/815

Introduction. E-cadherin aberrant expression or complete loss is common for a number of human malignant neoplasms, and can be a launching mechanism of an epithelial-mesenchymal transition. Passing through epithelial-mesenchymal transition could in turn promote to the acquisition of so called cancer stem cell phenotype by the transformed cells. The objective of the present study is to reveal the influence of E-cadherin expression level on the amount of cancer stem cells in human colon cancer cell line HCT116. Materials and methods. We have created cell sublines with E-cadherin up- and downregulation and assessed the percentage of cancer stem cells using tumor formation assay, clonogenic assay; we also evaluated profile of cell pluripotency markers. Results and conclusion. We have shown that the proportion of cancer stem cells in human colon adenocarcinoma cell line HCT116 depends on the E-cadherin expression level. E-cadherin expression downregulation results in elevated expression of pluripotency genes and in the increase of proportion of cancer stem cells via activation of Wnt/ß-signalling pathway. E-cadherin upregulation has a reverse effect and decreases the amount of HCT116 cancer stem cells. Thus, E-cadherin expression restoration seems prospective in colorectal anticancer therapy.

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