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<article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:ali="http://www.niso.org/schemas/ali/1.0/" article-type="other" dtd-version="1.2" xml:lang="en"><front><journal-meta><journal-id journal-id-type="publisher-id">Russian Journal of Biotherapy</journal-id><journal-title-group><journal-title xml:lang="en">Russian Journal of Biotherapy</journal-title><trans-title-group xml:lang="ru"><trans-title>Российский биотерапевтический журнал</trans-title></trans-title-group></journal-title-group><issn publication-format="print">1726-9784</issn><issn publication-format="electronic">1726-9792</issn><publisher><publisher-name xml:lang="en">Publishing House ABV Press</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="publisher-id">812</article-id><article-id pub-id-type="doi">10.17650/1726-9784-2016-15-2-76-81</article-id><article-categories><subj-group subj-group-type="toc-heading" xml:lang="en"><subject>ORIGINAL REPORTS</subject></subj-group><subj-group subj-group-type="toc-heading" xml:lang="ru"><subject>ОРИГИНАЛЬНЫЕ СТАТЬИ</subject></subj-group><subj-group subj-group-type="article-type"><subject></subject></subj-group></article-categories><title-group><article-title xml:lang="en">New method of extraction of intact nuclei from cells for flow cytometry fluorescence immunoassay</article-title><trans-title-group xml:lang="ru"><trans-title>Новый метод выделения чистой фракции интактных клеточных ядер для иммунофлуоресцентного анализа на проточном цитофлуориметре</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author"><name-alternatives><name xml:lang="en"><surname>Nikulitskiy</surname><given-names>S. I.</given-names></name><name xml:lang="ru"><surname>Никулицкий</surname><given-names>С. И.</given-names></name></name-alternatives><address><country country="RU">Russian Federation</country></address><xref ref-type="aff" rid="aff1"/></contrib><contrib contrib-type="author"><name-alternatives><name xml:lang="en"><surname>Tyrsina</surname><given-names>E. G.</given-names></name><name xml:lang="ru"><surname>Тырсина</surname><given-names>Екатерина Григорьевна</given-names></name></name-alternatives><address><country country="RU">Russian Federation</country></address><email>tyrsina@yandex.ru</email><xref ref-type="aff" rid="aff1"/></contrib><contrib contrib-type="author"><name-alternatives><name xml:lang="en"><surname>Inshakov</surname><given-names>A. N.</given-names></name><name xml:lang="ru"><surname>Иншаков</surname><given-names>А. Н.</given-names></name></name-alternatives><address><country country="RU">Russian Federation</country></address><xref ref-type="aff" rid="aff1"/></contrib><contrib contrib-type="author"><name-alternatives><name xml:lang="en"><surname>Borovkova</surname><given-names>N. B.</given-names></name><name xml:lang="ru"><surname>Боровкова</surname><given-names>Н. Б.</given-names></name></name-alternatives><address><country country="RU">Russian Federation</country></address><xref ref-type="aff" rid="aff1"/></contrib></contrib-group><aff-alternatives id="aff1"><aff><institution xml:lang="en">N.N. Blokhin Russian Cancer Research Center, Ministry of Health of Russia</institution></aff><aff><institution xml:lang="ru">ФГБУ«Российский онкологический научный центр им. Н.Н. Блохина» Минздрава России</institution></aff></aff-alternatives><pub-date date-type="pub" iso-8601-date="2016-06-30" publication-format="electronic"><day>30</day><month>06</month><year>2016</year></pub-date><volume>15</volume><issue>2</issue><fpage>76</fpage><lpage>81</lpage><history><date date-type="received" iso-8601-date="2018-04-10"><day>10</day><month>04</month><year>2018</year></date></history><permissions/><self-uri xlink:href="https://bioterapevt.abvpress.ru/jour/article/view/812">https://bioterapevt.abvpress.ru/jour/article/view/812</self-uri><abstract xml:lang="en"><p/></abstract><trans-abstract xml:lang="ru"><p/></trans-abstract><kwd-group xml:lang="ru"><kwd>экстракция интактных ядер</kwd><kwd>проточная цитофлуориметрия</kwd><kwd>рецептор фактора роста эндотелия сосудов 1-го типа</kwd><kwd>клеточная линия A431</kwd><kwd>extraction of nuclei</kwd><kwd>flow cytometry</kwd><kwd>receptor type 1 for vascular endothelial growth factor</kwd><kwd>A431 cell line</kwd></kwd-group></article-meta></front><body></body><back><ref-list><ref id="B1"><label>1.</label><mixed-citation>Георгиев Г.П., Мантьева В.Л. 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