Russian Journal of BiotherapyRussian Journal of BiotherapyРоссийский биотерапевтический журнал1726-97841726-9792Publishing House ABV Press4010.17650/1726-9784-2015-14-4-31-38ЭКСПЕРИМЕНТАЛЬНЫЕ ИССЛЕДОВАНИЯANALYTICAL CHARACTERISTICS OF FLUORESCENT SUSPENSION NANOCRYSTAL-ENCODED MICROARRAY ADAPTED FOR SIMULTANEOUS QUANTITATIVE DETECTION OF FREE AND TOTAL PSA IN SERUM SAMPLESАНАЛИТИЧЕСКИЕ ХАРАКТЕРИСТИКИ СУСПЕНЗИОННОЙ СИСТЕМЫ НА ОСНОВЕ МИКРОСФЕР, КОДИРОВАННЫХ ФЛУОРЕСЦЕНТНЫМИ НАНОКРИСТАЛЛАМИ, ДЛЯ ОДНОВРЕМЕННОЙ КОЛИЧЕСТВЕННОЙ ДЕТЕКЦИИ СВОБОДНОГО И ОБЩЕГО ПСА В СЫВОРОТКЕ КРОВИ ЧЕЛОВЕКАBrazhnikK. I.БражникК. И.
Russian Federation
SokolovaZ. A.СоколоваЗ. А.
Russian Federation
BaryshnikovaM. A.БарышниковаМ. А.
Russian Federation
BilanR. S.БиланР. С.
Russian Federation
NabievI. R.НабиевИ. Р.
Russian Federation
SukhanovaA. V.СухановаАлена Владимировна
Russian Federation
alvona.sukhanova@univ-reims.frNational Research Nuclear University «Moscow Engineering Physics Institute»Национальный Исследовательский Ядерный Университет «МИФИ»FSBI «N.N. Blokhin Russian Cancer Research Center»ФГБУ «РОНЦ им. Н.Н.Блохина»Université de ReimsУниверситет г. Реймс30122015144313810042018https://bioterapevt.abvpress.ru/jour/article/view/40

Multiplexed suspension systems of anew generation are capable to provide precise quantitative profiling of mul- tipledisease-specific markers in human body fluids. We have developed suspension microarrays based on microbeads encoded with fluorescent nanociystals, that show undeniable advantages overavailable analogues in terms of improved multiplexing capabilities, physical and optical properties, low cost and simplicity of analysisperformance. We have adapted QD-encoded suspension microarrays for the simultaneous detection of two forms of prostate-specific antigen (PSA) in human serum by means of classical flow cytometiy. In the present study, we describe in detail the designed system properties including evaluation the microarray for quantitative analysis of serum markers in comparison to standard clinical approach ELISA, as well asestimation of the most important analytical characteristics, such as analytic sensitivity (limit of detection), reproducibility, reliability and accuracy of the analysis, the linear rangesfor detectable- marker concentrations. Experimental data suggested that the developed diagnostic system quantifies two forms of PSA in blood serum samples of patients with high accuracy, precision and reliability.

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