Russian Journal of BiotherapyRussian Journal of BiotherapyРоссийский биотерапевтический журнал1726-97841726-9792Publishing House ABV Press1810.17650/1726-9784-2015-14-2-53-58ORIGINAL REPORTSОРИГИНАЛЬНЫЕ СТАТЬИINHIBITION OF MT1-MMP (MMP14) IMPROVES ANT-GLIOMA EFFECT OF COMBINATION: TEMOZOLOMIDE-IONIZING RADIATION IN THE GLIOBLASTOMA CANCER CELLSПОДАВЛЕНИЕ ЭКСПРЕССИИ КЛЕТОЧНОЙ МЕТАЛЛОПРОТЕИНАЗЫ ТИП 14 УСИЛИВАЕТ ПРОТИВООПУХОЛЕВЫЙ ЭФФЕКТ ТЕМОЗОЛОМИДА И ИОНИЗИРУЮЩЕЙ РАДИАЦИИ В КЛЕТКАХ ГЛИОБЛАСТОМ ЧЕЛОВЕКАUlasovI. V.УласовИлья Валентинович
Russian Federation
ulasov75@yahoo.comKaverinaN. V.КаверинаН. В.
Russian Federation
KadagidzeZ. G.КадагидзеЗ. Г.
Russian Federation
BaryshnikovA. Y.БарышниковА. Ю.
Russian Federation
Центр опухолей головного мозга, Шведский медицинский центрФГБНУ «РОНЦ им Н.Н. Блохина»30062015142535810042018https://bioterapevt.abvpress.ru/jour/article/view/18

The main goal of our study is to provide clinical and biological assessment of MMP14 as gene that causes recurrent glioblastoma. We analyzed expression of MMP14 in 108 and 129 samples represented by Rembrandt 8РOncomine databases. The biological significance was done is based on genetic knockdown and rescuing of MMP14 attenuated U87 and U251 glioma cells followed by cytofluorimetry and array gene expression. As expected, genetic silencing of MMP14 led to accumulation cells in the “G2” phase of cell divisions which correlates to cell kinase’s expression. Furthermore, exposure of MMP14 to ionizing radiation and temozolomidt treatments prolongs glioma cell proliferation and increase the anti-glioma effect of temozolomide-ionizing radiation therapeutic combination. Our data suggest that most of the neoplastic glioma cells express significantly MMP14.Therefore, alteration of MMP14, improves anti-tumor effect.

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