Russian Journal of BiotherapyRussian Journal of BiotherapyРоссийский биотерапевтический журнал1726-97841726-9792Publishing House ABV Press126110.17650/1726-9784-2021-20-2-76-84ORIGINAL REPORTSОРИГИНАЛЬНЫЕ СТАТЬИTHE EFFECT OF MULTIAXIAL DEFORMATION ON THE DYNAMICS OF BIODEGRADATION AND CELL COLONIZATION OF ALLOY WE43ВЛИЯНИЕ МУЛЬТИОСЕВОЙ ДЕФОРМАЦИИ НА ДИНАМИКУ БИОДЕГРАДАЦИИ СПЛАВА WE43 И КОЛОНИЗАЦИЮ КЛЕТКАМИhttps://orcid.org/0000-0003-1662-1904MartynenkoN. S.МартыненкоН. С.
Russian Federation

Natalia Sergeevna Martynenko

49 Leninsky Ave., Moscow 119334

Наталья Сергеевна Мартыненко

119334 Москва, Ленинский пр-т, 49

nataliasmartynenko@gmail.comhttps://orcid.org/0000-0002-4370-6578AnisimovN. Yu.АнисимоваН. Ю.
Russian Federation

24 Kashyrskoe Shosse, Moscow 115478

115478 Москва, Каширское шоссе, 24

https://orcid.org/0000-0002-0773-255XNovruzovK. M.НоврузовК. M.
Russian Federation

24 Kashyrskoe Shosse, Moscow 115478

115478 Москва, Каширское шоссе, 24

https://orcid.org/0000-0003-4232-927XDobatkinS. V.ДобаткинС. В.
Russian Federation

49 Leninsky Ave., Moscow 119334

119334 Москва, Ленинский пр-т, 49

https://orcid.org/0000-0002-0132-167XKiselevskiyM. V.КиселевскийМ. В.
Russian Federation

24 Kashyrskoe Shosse, Moscow 115478

115478 Москва, Каширское шоссе, 24

https://orcid.org/0000-0001-7784-5704EstrinYu. Z.ЭстринЮ. З.
Australia

Wellington Road, Clayton, Victoria 3800

35 Stirling Highway, Perth, Western Australia 6009

3800 Виктория, Клейтон, Веллингтон-Роуд

6009 Западная Австралия, Перт, Стирлинг шоссе, 35

A.A. Baikov Institute of Metallurgy and Materials Science, Russian Academy of SciencesИнститут металлургии и материаловедения им. А. А. Байкова РАНN.N. Blokhin National Medical Research Center of Oncology, Ministry of Health of RussiaФГБУ «Национальный медицинский исследовательский центр онкологии им. Н. Н. Блохина» Минздрава РоссииMonash University, Department of Materials Science and EngineeringУниверситет им. Монаша, Департамент материаловедения и инжинирингаUniversity of Western Australia, Department of Mechanical EngineeringУниверситет Западной Австралии, Департамент машиностроения1407202120276841407202114072021https://bioterapevt.abvpress.ru/jour/article/view/1261

Introduction. The development of materials for bioresorbable implants is an urgent issue in medicine and materials science. Magnesium alloys are promising materials for this purpose. In particular, alloy WE43 (Mg-Y-Nd-Zr) has proven itself well in this field. However, the use of magnesium alloys is limited by a high degradation rate, which is often accompanied with nonuniform corrosion, which negatively affects the load bearing capacity of the product. In addition, the increased degradation rate usually seriously worsens the biocompatibility of magnesium alloys. Therefore, the study of the corrosion resistance of magnesium alloys, as well astheir biocompatibility, is an urgent task.Purpose of the study was to investigate the effect of multiaxial deformation (MAD), aimed at increasing the mechanical characteristics of the alloy WE43, on its biodegradation kinetics, as well as on cell colonization.Materials and methods. The alloy WE43 in two states  – homogenized (WE43 hom) and strengthened by MAD (WE43 MAD) was investigated in this work. The kinetics of biodegradation was investigated on an xCELLigence RTCA Systems analyzer. A method for estimating the volume of hydrogen was used to study the process of gas formation, which was recorded using an automated digital microscope LionheartTM FX. The corrosive medium was a solution based on Dulbecco’s Modified Eagle’s Medium. A culture of mesenchymal multipotent stromal cells was used to study the colonization of the alloy surface by cells.Results. MAD of the alloy WE43 leads to a decrease in the biodegradation rate and the intensity of gas formation. The period of stabilization of biodegradation for the alloy after the MAD is 16 hours versus 3 hours for the alloy after homogenization. In this case, the volume of released hydrogen was 65.0 ± 4.4  mm3H2/mm3 alloy and 211.0 ± ± 21.1 mm3H2/mm3 alloy for the alloy after MAD and homogenization, respectively. MAD improves the biocompatibility of the alloy WE43, stimulating the colonization of mesenchymal multipotent stromal cells.Conclusion. MAD reduces biodegradation and improves the biocompatibility of the alloy WE43, which makes it a promising medical material, including for the purposes of oncoorthopedics

Введение. Разработка материалов для создания биорезорбируемых имплантатов – актуальный вопрос медицины и материаловедения. Перспективными материалами для данной цели являются магниевые сплавы, в частности сплав WE43 (Mg-Y-Nd-Zr), хорошо зарекомендовавший себя в этой области. Однако применение магниевых сплавов ограничено высокой скоростью деградации, которая часто сопровождается неравномерностью коррозии, что негативно сказывается на несущей способности изделия. Кроме того, повышенная скорость деградации может серьезно ухудшать биосовместимость магниевых сплавов. Поэтому изучение коррозионной стойкости магниевых сплавов, а также их биосовместимости является актуальной задачей.Цель исследования – изучить влияние мультиосевой деформации (МОД), направленной на повышение механических характеристик сплава WE43, на кинетику его биодеградации, а также на колонизацию клетками.Материалы и методы. В работе был исследован сплав WE43 в 2 состояниях: гомогенизированном (WE43 hom) и упрочненном методом МОД (WE43 MAD). Кинетику биодеградации исследовали на анализаторе xCELLigence RTCA Systems. Для  исследования газообразования был применен метод оценки объема водорода, который определяли с  помощью автоматизированного цифрового микроскопа LionheartTM FX. Коррозионной средой выступал раствор на основе Dulbecco’s Modified Eagle’s Medium. Для изучения колонизации поверхности сплава клетками использовали культуру мезенхимальных мультипотентных стромальных клеток.Результаты. МОД сплава WE43 приводит к снижению скорости биодеградации и интенсивности газообразования. Период стабилизациибиодеградациидля сплава послеМОДсоставляет 16 ч против 3 ч для сплава после гомогенизации. При этом объем выделившегося водорода составил 65,0 ± 4,4 мм3 H2 /мм3 сплава и 211,0 ± 21,1 мм3 H2 /мм3 сплава для материала после МОД и гомогенизации соответственно. МОД улучшает биосовместимость сплава WE43, стимулируя колонизацию мезенхимальными мультипотентными стромальными клетками.Заключение. МОД уменьшает биодеградацию и улучшает биосовместимость сплава WE43, что делает его перспективным медицинским материалом, в том числе для целей онкоортопедии.

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