ANTI-TUMOR EFFECT OF CPDA ENANTIOMERS IN VITRO IN THE MODEL OF ACUTE LYMPHOBLASTIC LEUKEMIA

A. V. Savinkova; Савинкова А. В.; L. R. Tilova; Тилова Л. Р.; O. I. Borisova; Борисова О. И.; E. M. Zhidkova; Жидкова Е. М.; K. A. Kuzin; Кузин К. А.; K. I. Kirsanov; Кирсанов К. И.; G. A. Belitsky; Белицкий Г. А.; I. V. Budunova; Будунова И. В.; M. G. Yakubovskaya; Якубовская М. Г.; E. A. Lesovaya; Лесовая Екатерина Андреевна

doi:10.17650/1726-9784-2017-16-1-61-69

ANTI-TUMOR EFFECT OF CPDA ENANTIOMERS IN VITRO IN THE MODEL OF ACUTE LYMPHOBLASTIC LEUKEMIA

Authors: Savinkova A.V.¹, Tilova L.R.¹, Borisova O.I.¹, Zhidkova E.M.¹^,2, Kuzin K.A.¹, Kirsanov K.I.¹, Belitsky G.A.¹, Budunova I.V.³, Yakubovskaya M.G.¹, Lesovaya E.A.¹^,4
Affiliations:
1. N.N. Blokhin Russian Cancer Research Center
2. Moscow Technological University
3. Northwestern University
4. Ryazan State Medical University named after academician I.P. Pavlov
Issue: Vol 16, No 1 (2017)
Pages: 61-69
Section: ORIGINAL REPORTS
Published: 30.03.2017
URL: https://bioterapevt.abvpress.ru/jour/article/view/856
DOI: https://doi.org/10.17650/1726-9784-2017-16-1-61-69
ID: 856

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Abstract

Introduction. Glucocorticoids are the important component of combined chemotherapy of blood cancer. Therapeutic effects of glucocorticoids is realized via activation of glucocorticoid receptor transrepression, the development of side effects is associated with transactivation. We demonstrated earlier that compound belonging the class of selective glucocorticoid receptor agonists, CpdA, selectively induced transrepression in blood cancer cells. CpdA represents a mixture of two enantiomers, which can differ in interaction with the receptor. Aim. The main aim of present study was to synthesize CpdA enantiomers and to evaluate their biological properties. Materials and methods. Synthesis was carried out based on Sharpless dihydroxylation; anti-tumor activity in vitro was evaluated by antiproliferative and pro-apoptotic effects. Ligand properties were estimated by PCR-analysis of glucocorticoid- and NF-kB-dependent genes expression. Results and conclusions. We demonstrated that CpdA enantiomers revealed anti-tumor activity in vitro and did not induce transactivation. Moreover, S-enantiomer of CpdA in the most tests demonstrated more pronounced activity and is more perspective molecule for future studies in vivo.

Keywords

глюкокортикоидный рецептор, энантиомеры, гемобластозы, транс-активация, транс-репрессия, противоопухолевая активность, glucocorticoid receptor, enantiomers, hemoblastosis, transactivation, transrepression, anti-tumor activity

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ANTI-TUMOR EFFECT OF CPDA ENANTIOMERS IN VITRO IN THE MODEL OF ACUTE LYMPHOBLASTIC LEUKEMIA

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Abstract

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About the authors

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