EVALUATION OF BIOMODIFIED L-ASPARAGINASE WAS79 SIDE EFFECTS IN CHRONIC EXPERIMENT ON RABBITS

E. R. Pereverzeva; Переверзева Элеонора Рафаиловна; I. D. Treschalin; Трещалин И. Д.; M. I. Treschalin; Трещалин М. И.; E. V. Voznyakovskaya; Возняковская Е. В.; T. B. Pereverzeva; Переверзева Т. Б.; N. V. Eremkin; Еремкин Н. В.; N. V. Bulushova; Булушова Н. В.; E. P. Sannikova; Санникова Е. П.

doi:10.17650/1726-9784-2016-15-3-47-54

EVALUATION OF BIOMODIFIED L-ASPARAGINASE WAS79 SIDE EFFECTS IN CHRONIC EXPERIMENT ON RABBITS

Authors: Pereverzeva E.R.¹, Treschalin I.D.¹, Treschalin M.I.¹, Voznyakovskaya E.V.¹, Pereverzeva T.B.¹, Eremkin N.V.¹, Bulushova N.V.², Sannikova E.P.²
Affiliations:
1. ФГБНУ «Научно-исследовательский институт по изысканию новых антибиотиков имени Г.Ф. Гаузе»
2. ФГУП«Государственный научно-исследовательский институт генетики и селекции промышленных микроорганизмов»
Issue: Vol 15, No 3 (2016)
Pages: 47-54
Section: ORIGINAL REPORTS
Published: 30.09.2016
URL: https://bioterapevt.abvpress.ru/jour/article/view/821
DOI: https://doi.org/10.17650/1726-9784-2016-15-3-47-54
ID: 821

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Abstract

Objective. The aim of the study was to research the toxicity of L-asparaginase Was79 in rabbits. Materials and methods. Chronic toxicity of L-asparaginase Was79, obtained by modification of native enzyme Wolinella succinogenes in Research Institute of Genetics and Selection, was performed in male and female Russian chinchilla rabbits. L-asparaginase was injected intravenously at the 1 and 5 therapeutic dose (15 x 100 IU/kg or 15 x 500 IU/kg with 24-h interval). The following parameters were tested: body mass, clinical and biochemical blood tests, urinalysis, electrocardiography, pathomorphological evaluation of internal organs. Resutls. The results of the study suggest that the treatment with L-asparaginase Was79 does not influence on the function of heart and kidneys, but damages their structure. Loss in body mass, diarrhea and alteration of stomach and intestine mucosa could be interpreted as evidence of gastrointestinal toxicity. Hematological toxicity was exhibited as a decrease of total leukocyte count, lymphocyte and neutrophils count level in peripheral blood and atrophy of lymphoid tissue of the spleen, thymus and lymph nodes. Elevation of total and direct bilirubin in serum and histopathological findings in liver were found in groups treated with both high and low doses of Was79. Conclusion. Most of these abnormalities were reversible and dose-dependent.

Keywords

L-аспарагиназа, Wolinella succinogenes, хроническая токсичность, кролики, L-asparaginase, Wolinella succinogenes, chronic toxicity, rabbits

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EVALUATION OF BIOMODIFIED L-ASPARAGINASE WAS79 SIDE EFFECTS IN CHRONIC EXPERIMENT ON RABBITS

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Abstract

Keywords

About the authors

References

Supplementary files