Ginsenosides definition in plant extracts by means of high through liquid chromatography with tandem mass-spectrometry

R. V. Karpova; Карпова Регина Васильевна; V. E. Shevchenko; Шевченко В. Е.; E. V. Bocharov; Бочаров Е. В.; O. P. Sheychenko; Шейченко О. П.; O. A. Bocharova; Бочарова О. А.; V. G. Kucheryanu; Кучеряну В. Г.; V. A. Bykov; Быков В. А.

doi:10.17650/1726-9784-2016-15-2-36-46

Ginsenosides definition in plant extracts by means of high through liquid chromatography with tandem mass-spectrometry

Authors: Karpova R.V.¹, Shevchenko V.E.¹, Bocharov E.V.¹, Sheychenko O.P.², Bocharova O.A.¹, Kucheryanu V.G.³, Bykov V.A.²
Affiliations:
1. N.N. Blokhin Russian Cancer Research Center, Ministry of Health of Russia
2. All-Russian Research Institute of Medical and Aromatic plants
3. Institute of general pathology and pathophysiology
Issue: Vol 15, No 2 (2016)
Pages: 36-46
Section: ORIGINAL REPORTS
Published: 30.06.2016
URL: https://bioterapevt.abvpress.ru/jour/article/view/807
DOI: https://doi.org/10.17650/1726-9784-2016-15-2-36-46
ID: 807

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Abstract

Background. Present study was dedicated to identification and evaluation of the biologically active components in multiphytoadapto-gene preparation phytomix-40 (PhM-40). It consists of forty plant extracts components including Panax ginseng. PhM-40 demonstrates wide spectrum of biological effects including antimutagenic, antitumor, radioprotective, hormone-modulating, antioxidant, neuropro-tective and ummunomodulating activities. Objective: of this study was to identify and to quantify ginsenosides content in the preparation. Materials and method. Experimental design included the comparison of ginsenosides content evaluation in PhM-40, comparison preparation (which contains similar to PhM-40 plant extracts components but another rations) and Panax ginseng extract by means of LC-MS/MS spectroscopy method. Two different gradients were used: the short and the long ones. By means of the short one rapid compound identification with economical consumption of chemicals was possible as well as the long one allowed us to identify compounds with better resolution capability. Commercially available ginsenosides were the standards for calibration. Literature data were also used for ginsenosides identification. Results. High through liquid chromatography method with tandem mass-spectrometry was successfully used for ginsenosides identification quantitatively and qualitatively in plant preparation PhM-40. The data obtained show the ginsenosides qualitative composition which has been identified as Rb, Rb₂, Rc, Rd, Rg_p Rg₂, Re, Rf, Ro, malonyl-Rb_p -Rb₂/Rb₃/Rc, -Rd enumeration in PhM-40

Keywords

фитоадаптоген, гинзенозиды, электрораспыление, тандемная масс-спектрометрия, пределы детектирования, phytoadaptogene, ginsenosides, electro spray, tandem mass-spectrometry, limits of detection

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Ginsenosides definition in plant extracts by means of high through liquid chromatography with tandem mass-spectrometry

Full Text

Abstract

Keywords

About the authors

R. V. Karpova

V. E. Shevchenko

E. V. Bocharov

O. P. Sheychenko

O. A. Bocharova

V. G. Kucheryanu

V. A. Bykov

References

Supplementary files