DEVELOPING A MODEL OF LIPOSOMAL FORMULATION OF A NEW NATIONAL ANALOGUE HYPOTHALAMIC HORMONE SOMATOSTATIN, WHICH HAS ANTI-TUMOR ACTIVITY

E. V. Sanarova; Санарова Екатерина Викторовна; A. V. Lantsova; Ланцова А. В.; Xi Zhang; Чжан Си; M. V. Dmitrieva; Дмитриева М. В.; A. P. Polozkova; Полозкова А. П.; O. L. Orlova; Орлова О. Л.; Z. S. Shprakh; Шпрах З. С.; M. P. Kiseleva; Киселева М. П.; L. M. Borisova; Борисова Л. М.; Z. S. Smirnova; Смирнова З. С.; N. A. Oborotova; Оборотова Н. А.

doi:10.17650/1726-9784-2015-14-4-73-78

DEVELOPING A MODEL OF LIPOSOMAL FORMULATION OF A NEW NATIONAL ANALOGUE HYPOTHALAMIC HORMONE SOMATOSTATIN, WHICH HAS ANTI-TUMOR ACTIVITY

Authors: Sanarova E.V.¹, Lantsova A.V.¹, Zhang X.², Dmitrieva M.V.¹, Polozkova A.P.¹, Orlova O.L.¹, Shprakh Z.S.¹, Kiseleva M.P.¹, Borisova L.M.¹, Smirnova Z.S.¹, Oborotova N.A.¹
Affiliations:
1. FSBI «N.N. Blokhin Russian Cancer Research Center» of the Ministry of Health of the Russian Federation
2. I.M. Sechenov First Moscow State Medical University
Issue: Vol 14, No 4 (2015)
Pages: 73-78
Section: ЭКСПЕРИМЕНТАЛЬНЫЕ ИССЛЕДОВАНИЯ
Published: 30.12.2015
URL: https://bioterapevt.abvpress.ru/jour/article/view/46
DOI: https://doi.org/10.17650/1726-9784-2015-14-4-73-78
ID: 46

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Abstract

At present, the hormone somatostatin analogues is increasing interest in connection with their activity against hormone-dependent tumors, which leads to the need to develop domestic drugs belonging to this group. In the laboratory of chemical synthesis Institute of experimental diagnostics and chemotherapy of FSBI «N.N. Blokhin RCRC» synthesized new domestic pentapeptide somatostatin analogue of hypothalamic hormone (AGGS). In preliminary studies of this substance demonstrated sufficiently high antitumor activity AGGS on transplanted solid tumors of mice. Due to the insolubility of the substance in the water as an alternative liposomal formulation proposed, allowing to increase the bioavailability of the drag due to the possibility of intravenous administration, increased therapeutic efficacy and reduce side effects, by improving the selectivity of action against tumor cells. During experiments on the development of the liposomal formulation AGGS pre-established model containing as essential components of the liposomal bilayer and egg lecithin PEG-2000-DSPE in a molar ratio 72/1 and as a ciyo- protectant - sucrose. In developing the technological parameters of the process of obtaining dosage form (LF) found that for an acceptable size derived phosphohpid vesicles needs about 7 cycles extrusion. The lack of stability of liposomal dispersion selected composition during storage has led to this problem by means of freeze-drying, which kept the physico-chemical parameters LF at the initial level. The efficacy of the model LF on transplantable tumors of mice - breast adenocarcinoma Ca-755, which was more than 60 % of ITG at a dose of 5 mg / kg and more than 80 % ITG with 20 mg / kg. The findings of biological experiments indicate the prospects for further research and improvement liposomal LF to produce high-performance domestic anticancer drag from the group of somatostatin analogues.

Keywords

аналог гипоталамического гормона соматостатина, липосомы, лекарственная форма, технология получения, hypothalamic hormone somatostatin analogue, liposomes, dosage form, technology of production

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DEVELOPING A MODEL OF LIPOSOMAL FORMULATION OF A NEW NATIONAL ANALOGUE HYPOTHALAMIC HORMONE SOMATOSTATIN, WHICH HAS ANTI-TUMOR ACTIVITY

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Abstract

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About the authors

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