ANALYTICAL CHARACTERISTICS OF FLUORESCENT SUSPENSION NANOCRYSTAL-ENCODED MICROARRAY ADAPTED FOR SIMULTANEOUS QUANTITATIVE DETECTION OF FREE AND TOTAL PSA IN SERUM SAMPLES

K. I. Brazhnik; Бражник К. И.; Z. A. Sokolova; Соколова З. А.; M. A. Baryshnikova; Барышникова М. А.; R. S. Bilan; Билан Р. С.; I. R. Nabiev; Набиев И. Р.; A. V. Sukhanova; Суханова Алена Владимировна

doi:10.17650/1726-9784-2015-14-4-31-38

ANALYTICAL CHARACTERISTICS OF FLUORESCENT SUSPENSION NANOCRYSTAL-ENCODED MICROARRAY ADAPTED FOR SIMULTANEOUS QUANTITATIVE DETECTION OF FREE AND TOTAL PSA IN SERUM SAMPLES

Authors: Brazhnik K.I.¹, Sokolova Z.A.¹^,2, Baryshnikova M.A.¹^,2, Bilan R.S.¹, Nabiev I.R.¹^,3, Sukhanova A.V.¹^,3
Affiliations:
1. National Research Nuclear University «Moscow Engineering Physics Institute»
2. FSBI «N.N. Blokhin Russian Cancer Research Center»
3. Université de Reims
Issue: Vol 14, No 4 (2015)
Pages: 31-38
Section: ЭКСПЕРИМЕНТАЛЬНЫЕ ИССЛЕДОВАНИЯ
Published: 30.12.2015
URL: https://bioterapevt.abvpress.ru/jour/article/view/40
DOI: https://doi.org/10.17650/1726-9784-2015-14-4-31-38
ID: 40

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Abstract

Multiplexed suspension systems of anew generation are capable to provide precise quantitative profiling of mul- tipledisease-specific markers in human body fluids. We have developed suspension microarrays based on microbeads encoded with fluorescent nanociystals, that show undeniable advantages overavailable analogues in terms of improved multiplexing capabilities, physical and optical properties, low cost and simplicity of analysisperformance. We have adapted QD-encoded suspension microarrays for the simultaneous detection of two forms of prostate-specific antigen (PSA) in human serum by means of classical flow cytometiy. In the present study, we describe in detail the designed system properties including evaluation the microarray for quantitative analysis of serum markers in comparison to standard clinical approach ELISA, as well asestimation of the most important analytical characteristics, such as analytic sensitivity (limit of detection), reproducibility, reliability and accuracy of the analysis, the linear rangesfor detectable- marker concentrations. Experimental data suggested that the developed diagnostic system quantifies two forms of PSA in blood serum samples of patients with high accuracy, precision and reliability.

Keywords

квантовые точки, простатический специфический антиген, суспензионные микрочипы, многопараметрический анализ, проточная цитометрия, fluorescent semiconductor nanocrystals, quantum dots, cancer markers, prostate-specific antigen, microbeads, suspension microarrays, multiplexed analysis, flow cytometry

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ANALYTICAL CHARACTERISTICS OF FLUORESCENT SUSPENSION NANOCRYSTAL-ENCODED MICROARRAY ADAPTED FOR SIMULTANEOUS QUANTITATIVE DETECTION OF FREE AND TOTAL PSA IN SERUM SAMPLES

Full Text

Abstract

Keywords

About the authors

K. I. Brazhnik

Z. A. Sokolova

M. A. Baryshnikova

R. S. Bilan

I. R. Nabiev

A. V. Sukhanova

References

Supplementary files