PRAME PROTEIN ARE LOCATED IN CELL NUCLEUS DURING ITS GENE IS HYPEREXPRESSED

N. A. Lyzhko; Лыжко Н. А.; A. V. Misyurin; Ахлынина Т. В.; T. V. Ahlynina; Мисюрин А. В.; Y. P. Finashutina; Финашутина Ю. П.; E. V. Aksenova; Аксенова Е. В.; I. N. Soldatova; Солдатова И. Н.; V. A. Misyurin; Мисюрин Всеволод Андреевич; A. Yu. Baryshnikov; Барышников А. Ю.

doi:10.17650/1726-9784-2015-14-4-19-30

PRAME PROTEIN ARE LOCATED IN CELL NUCLEUS DURING ITS GENE IS HYPEREXPRESSED

Authors: Lyzhko N.A.¹^,2, Misyurin A.V.¹^,2, Ahlynina T.V.¹^,2, Finashutina Y.P.¹^,2, Aksenova E.V.¹^,2, Soldatova I.N.¹^,2, Misyurin V.A.¹^,2, Baryshnikov A.Y.¹
Affiliations:
1. FSBI «N.N. Blokhin Russian Cancer Research Center»
2. «GeneTechnology» LLC
Issue: Vol 14, No 4 (2015)
Pages: 19-30
Section: ЭКСПЕРИМЕНТАЛЬНЫЕ ИССЛЕДОВАНИЯ
Published: 30.12.2015
URL: https://bioterapevt.abvpress.ru/jour/article/view/39
DOI: https://doi.org/10.17650/1726-9784-2015-14-4-19-30
ID: 39

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Abstract

The problem on the localization of the protein PRAME in cancer cell remains unclear. We have created two antibodies, that specifically recognize PRAME epitopes. Using them, we have checked the localization of PRAME protein in some cell lines, including K562 and mel Kor, that characterized by high PRAME gene expression level, original lines B16F10 and WI-38, and B16F10 and WI-38, that transfected by PRAME gene. Finally, we have investigated the PRAME localization in acute myeloid leukemia patient's bone marrow cells. We have shown, the PRAME protein are localisated in nucleus, cytoplasm and plasma membrane of cells with high PRAME gene expression levels. PRAME protein localisation only in cytoplasm and plasma membrane in cells with lower gene expression level. Moreover, our antibodies demonstrated cytotoxic effect during co-incubation with PRAME¹ cells.

Keywords

экспрессия гена FRAME, локализация в клетке, белок PRAME, PRAME gene expression, localization, protein PRAME

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PRAME PROTEIN ARE LOCATED IN CELL NUCLEUS DURING ITS GENE IS HYPEREXPRESSED

Full Text

Abstract

Keywords

About the authors

References

Supplementary files