MONOCLONAL ANTIBODIES ICO-401 AGAINST CD133 ANTIGEN

O. S. Burova; Бурова Ольга Семеновна; N. V. Golubtsova; Голубцова Н. В.; M. A. Baryshnikova; Бармашов А. Е.; M. V. Oborotova; Оборотова М. В.; L. F. Morozova; Морозова Л. Ф.; K. A. Baryshnikov; Барышников К. А.; A. E. Barmashov; Карасева В. И.; V. I. Karaseva; Барышникова М. А.; A. N. Inshakov; Иншаков А. Н.; A. S. Grinevich; Гриневич А. С.; I. M. Luchenko; Лученко И. М.; P. K. Ivanov; Иванов П. К.; A. Yu. Baryshnikov; Барышников А. Ю.

doi:10.17650/1726-9784-2015-14-3-37-40

MONOCLONAL ANTIBODIES ICO-401 AGAINST CD133 ANTIGEN

Authors: Burova O.S.¹, Golubtsova N.V.¹, Baryshnikova M.A.¹, Oborotova M.V.¹, Morozova L.F.¹, Baryshnikov K.A.¹, Barmashov A.E.¹, Karaseva V.I.¹, Inshakov A.N.¹, Grinevich A.S.¹, Luchenko I.M.¹, Ivanov P.K.¹, Baryshnikov A.Y.¹
Affiliations:
1. FSBI «N.N. Blokhin RCRC»
Issue: Vol 14, No 3 (2015)
Pages: 37-40
Section: ОРГИНАЛЬНЫЕ СТАТЬИ
Published: 30.09.2015
URL: https://bioterapevt.abvpress.ru/jour/article/view/31
DOI: https://doi.org/10.17650/1726-9784-2015-14-3-37-40
ID: 31

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Abstract

Monoclonal antibody (mAb) against CD133 antigen is a stem marker of human tumor cells. Strain ICO-401 was prepared by cell fusion of murine myeloma NS-1 cells with splenocytes of BALB/ C mice, pre-immunized three times with an interval of two weeks with the cells of human melanoma mel IbrEE34RMCR. Merging was conducted with solution PEG/DMSO. Screening of mAb ICO-401 was performed on human melanoma cell lines from FSBI «N.N. Blokhin RCRC» collection which differ in the expression of CD133 antigen. Antigen expression was evaluated in immunofluorescence reaction by flow cytometer BD FACSCanto^TMII. ICO-401 was compared with commercial mAb against CD133 antigen. The results indicated that both mAb recognize the same antigen, but bind to different epitops.

Keywords

моноклональные антитела, CD133, стволовые опухолевые клетки, меланома кожи человека, проточная цитометрия, monoclonal antibodies, CD133, tumor stem cells, human skin melanoma, flow сytometry

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MONOCLONAL ANTIBODIES ICO-401 AGAINST CD133 ANTIGEN

Full Text

Abstract

Keywords

About the authors

References

Supplementary files