New undifferentiated pleomorphic sarcoma cell line UPS134 for  in vitro  and  in vivo  studies

Polina A. Shtompel; Штомпель П. А.; Sofya A. Khazanova; Хазанова С. А.; Ekaterina S. Trapeznikova; Трапезникова Е. С.; Victoria Yu. Zinovieva; Зиновьева В. Ю.; Anastasia A. Lovenger; Ловенгер А. А.; Anastasia A. Tararykova; Тарарыкова А. А.; Beniamin Yu. Bokhyan; Бохян Б. Ю.; Natalia V. Khromova; Хромова Н. В.; Pavel B. Kopnin; Копнин П. Б.; Timur I. Fetisov; Фетисов Т. И.; Marianna G. Yakubovskaya; Якубовская М. Г.; Kirill I. Kirsanov; Кирсанов К. И.

doi:10.17650/1726-9784-2026-25-1-62-70

New undifferentiated pleomorphic sarcoma cell line UPS134 for in vitro and in vivo studies

Authors: Shtompel P.A.¹^,2, Khazanova S.A.¹^,2, Trapeznikova E.S.¹^,3, Zinovieva V.Y.¹, Lovenger A.A.¹, Tararykova A.A.¹, Bokhyan B.Y.¹, Khromova N.V.¹, Kopnin P.B.¹, Fetisov T.I.¹^,2, Yakubovskaya M.G.¹^,2, Kirsanov K.I.¹^,2
Affiliations:
1. N.N. Blokhin National Medical Research Center of Oncology, Ministry of Health of Russia
2. Patrice Lumumba Peoples’ Friendship University of Russia
3. Russian Сenter of Neurology and Neuroscience
Issue: Vol 25, No 1 (2026)
Pages: 62-70
Section: ORIGINAL REPORTS
Published: 30.04.2026
URL: https://bioterapevt.abvpress.ru/jour/article/view/1562
DOI: https://doi.org/10.17650/1726-9784-2026-25-1-62-70
ID: 1562

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Abstract
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Abstract

Background. Due to the limited number of cell lines, as well as the high heterogeneity of undifferentiated pleomorphic sarcomas (UPS), the creation of new stable cell lines is relevant.

Aim. Obtaining a stable cell line from a sample of UPS obtained during surgery to model carcinogenesis processes in vitro and in vivo.

Materials and methods. Cells were isolated from a tumor sample from a patient diagnosed with UPS. After passage 40 and stable growth, the proliferation rate, cell morphology, and growth ability in 3D culture conditions were assessed. To assess tumorigenicity, the cell suspension was injected into BALB / c nu / nu athymic mice. On the 15^thday of the experiment, the tumor was removed, its volume was measured, and it was examined histologically. Using short tandem repeat (STR) analysis, the cells of the resulting line were examined for the presence of human cell markers and their uniqueness. The chemosensitivity of the cells was assessed using the resazurin test.

Results. Stable growth and formation of spheroids under 3D culturing conditions were demonstrated for the cell culture. All mice inoculated with the UPS134 cell suspension showed tumor growth, and by day 14 of the experiment its average size was 28,7 mm³. Histologically, the tumor is similar in morphology to UPS, except for a smaller amount of myxoid and less polymorphism. The cells of the obtained line were shown to contain all analyzed genetic markers of human cells, and also that the STR data of the obtained line did not coincide with the STR of other lines. For doxorubicin, gemcitabine and docetaxel, IC₅₀ (inhibitory concentration) were determined to be 15, 6.5 and 10 % of the plasma peak concentration, respectively.

Conclusions. We obtained a new cell line UPS134, which can be further used to study the pathogenesis of UPS, as well as in preclinical studies of new antitumor agents in vitro and in vivo.

Keywords

undifferentiated pleomorphic sarcoma, cell line, assessing tumorigenicity

References

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