Email this article Study of Ridostin Pro and Poly(I:C) as adjuvants for cancer vaccine on the E.G7-OVA model
- Authors: Tsarapaev P.V.1, Baryshnikova M.A.1, Ponomarev A.V.1, Rudakova A.A.1, Sokolova Z.A.1, Baryshnikov K.A.1, Levagina G.M.2, Danilenko E.D.2, Kosorukov V.S.1
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Affiliations:
- N.N. Blokhin National Medical Research Center of Oncology, Ministry of Health of Russia
- Institute of Medical Biotechnology of the State Scientific Center of Virology and Biotechnology “Vektor”
- Issue: Vol 23, No 2 (2024)
- Pages: 78-84
- Section: ORIGINAL REPORTS
- Published: 26.06.2024
- URL: https://bioterapevt.abvpress.ru/jour/article/view/1453
- DOI: https://doi.org/10.17650/1726-9784-2024-23-2-78-84
- ID: 1453
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Abstract
Background. Adjuvant is necessary for enhancing the efficacy of cancer peptide vaccines. Our previous work has demonstrated the efficacy of TRL-3 agonists, which include Poly(I:C) and Ridostin Pro, as part of peptide neoantigen vaccines against murine melanoma B16-F10.
Aim. To evaluate the antitumor efficacy of Ridostin Pro or Poly(I:C) against murine lymphoma E.G7-OVA.
Materials and methods. The study was performed on C57Bl / 6 mice with subcutaneously transplanted E.G7-OVA lymphoma containing the complete chicken ovalbumin sequence. The antitumor effects of Ridostin Pro and Poly(I:C) were evaluated in monotherapy as well as in vaccines containing chicken ovalbumin in addition to the adjuvant. The antitumor effect of Ridostin Pro and Poly(I:C) was evaluated when used in different vaccination regimens: in one case treatment was started after tumour transplantation and in the other case before tumour transplantation. The criteria of antitumor response were inhibition of tumour growth, increased survival of mice and cure.
Results. Ridostin Pro and Poly(I:C) both as part of the vaccine and when administered without ovalbumin increased the percentage of tumour growth inhibition and survival of mice with E.G7-OVA lymphoma. In a regime where vaccination with ovalbumin and Ridostin Pro or Poly(I:C) was started before tumour transfection, a complete cure of the mice was shown.
Conclusion. Ridostin Pro and Poly(I:C) enhance the antitumor effect of a peptide vaccine against E.G7-OVA lymphoma.
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About the authors
P. V. Tsarapaev
N.N. Blokhin National Medical Research Center of Oncology, Ministry of Health of Russia
ORCID iD: 0000-0002-1182-1010
Pavel V. Tsarapaev
24 Kashirskoe Shosse, Moscow 115522
Russian FederationM. A. Baryshnikova
N.N. Blokhin National Medical Research Center of Oncology, Ministry of Health of Russia
Author for correspondence.
Email: maba@ronc.ru
ORCID iD: 0000-0002-6688-8423
Maria A. Baryshnikova
24 Kashirskoe Shosse, Moscow 115522
Russian FederationA. V. Ponomarev
N.N. Blokhin National Medical Research Center of Oncology, Ministry of Health of Russia
ORCID iD: 0000-0001-9517-8183
Aleksandr V. Ponomarev
24 Kashirskoe Shosse, Moscow 115522
Russian FederationA. A. Rudakova
N.N. Blokhin National Medical Research Center of Oncology, Ministry of Health of Russia
ORCID iD: 0000-0001-7266-7689
Anna A. Rudakova
24 Kashirskoe Shosse, Moscow 115522
Russian FederationZ. A. Sokolova
N.N. Blokhin National Medical Research Center of Oncology, Ministry of Health of Russia
ORCID iD: 0000-0003-4755-5313
Zinaida A. Sokolova
24 Kashirskoe Shosse, Moscow 115522
Russian FederationK. A. Baryshnikov
N.N. Blokhin National Medical Research Center of Oncology, Ministry of Health of Russia
ORCID iD: 0000-0002-9532-4264
Kirill A. Baryshnikov
24 Kashirskoe Shosse, Moscow 115522
Russian FederationG. M. Levagina
Institute of Medical Biotechnology of the State Scientific Center of Virology and Biotechnology “Vektor”
ORCID iD: 0000-0002-6749-5074
Galina M. Levagina
9 Khimzavodskaya St., Berdsk, Novosibirsk region 630010
Russian FederationE. D. Danilenko
Institute of Medical Biotechnology of the State Scientific Center of Virology and Biotechnology “Vektor”
ORCID iD: 0000-0001-5026-1602
Elena D. Danilenko
9 Khimzavodskaya St., Berdsk, Novosibirsk region 630010
Russian FederationV. S. Kosorukov
N.N. Blokhin National Medical Research Center of Oncology, Ministry of Health of Russia
ORCID iD: 0000-0002-8462-2178
Vyacheslav S. Kosorukov
24 Kashirskoe Shosse, Moscow 115522
Russian FederationReferences
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