Schisandrin and Schisantherin A in Multiphytoadaptogen for Preventive Oncology

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Abstract

Background. The original herbal formula of Multiphytoadaptogen (MPhA) for preventive oncology developed by the N. N. Blokhin Center of Oncology containing phytocomponents from Schisandra chinensis (Turcz.) Baill (Schisandraceae), has been investigated in vitro, in vivo. Preliminary efficiency in clinical trials has also been obtained. This was allowed because MPhA in Russia is registered as a parapharmaceutical agent and therefore standardized according to established requirements. However, due to the high efficiency of MPhA, a detailed study of the chemical composition and standardization of it is required, including the Schisandra chinensis active components, which turned out to be translocated into MPhA as a result of the extraction technology developed.

The aim of the study was to identify the Schisandra chinensis biologically active substances in MPhA and to evaluate the biological activity profiles of the identified phytocomponents using in silico analysis.

Materials and methods. we used high performance liquid chromatography in combination with mass spectrometry (HPLC–MS / MS). Chromatography was performed on an ACQUITY UPLC BEH C18 column in a gradient mode. A TSQ Vantage triple quadrupole mass spectrometer with electrospray ionization was used. we performed in silico analyzes of Schisandrin and Schisantherin A biological activity spectra using computer programs PASS and PharmaExpert.

Result. The secondary metabolites lignans Schisandrin and Schisantherin A were identified in the herbal formula MPhA. Schisandrin and Schisantherin A activities, according the scientific literature and in silico analysis, correspond to the properties studied for MPhA which therefore fits into the concept of medication for preventive oncology.

Conclusion. The determined secondary metabolites can be used for identification, standardization and quality testing of the herbal formula MPhA.

About the authors

O. A. Bocharova

N. N. Blokhin National Medical Research Center of Oncology, Ministry of Health of Russia

Author for correspondence.
ORCID iD: 0000-0002-6365-2888

24 Kashirskoe Shosse, Moscow115522

Russian Federation

I. V. Kazeev

N. N. Blokhin National Medical Research Center of Oncology, Ministry of Health of Russia

ORCID iD: 0000-0002-6100-3470

24 Kashirskoe Shosse, Moscow115522

Russian Federation

V. E. Shevchenko

N. N. Blokhin National Medical Research Center of Oncology, Ministry of Health of Russia

ORCID iD: 0000-0002-0401-9900

24 Kashirskoe Shosse, Moscow115522

Russian Federation

N. S. Ionov

Institute of Biomedical Chemistry

ORCID iD: 0000-0001-5396-2789

10 / 8 Pogodinskaya St., Moscow19121

Russian Federation

O. P. Sheichenko

All-Russian Scientific Research Institute of Medical and Aromatic Plants

ORCID iD: 0000-0001-7099-4192

7 / 1 Greena St., Moscow 117216

Russian Federation

E. V. Bocharov

N. N. Blokhin National Medical Research Center of Oncology, Ministry of Health of Russia

ORCID iD: 0000-0003-2342-9881

24 Kashirskoe Shosse, Moscow115522

Russian Federation

R. V. Karpova

N. N. Blokhin National Medical Research Center of Oncology, Ministry of Health of Russia

ORCID iD: 0000-0003-4893-1472

24 Kashirskoe Shosse, Moscow115522

Russian Federation

V. G. Kucheryanu

Institute of General Pathology and Pathophysiology

ORCID iD: 0000-0002-5071-3581

8 Baltiyskaya St., Moscow 125315

Russian Federation

A. A. Lagunin

Institute of Biomedical Chemistry

ORCID iD: 0000-0003-1757-8004

10 / 8 Pogodinskaya St., Moscow19121

Russian Federation

D. A. Filimonov

Institute of Biomedical Chemistry

ORCID iD: 0000-0002-0339-8478

10 / 8 Pogodinskaya St., Moscow19121

Russian Federation

V. S. Kosorukov

N. N. Blokhin National Medical Research Center of Oncology, Ministry of Health of Russia

ORCID iD: 0000-0002-8462-2178

24 Kashirskoe Shosse, Moscow115522

Russian Federation

V. V. Poroikov

Institute of Biomedical Chemistry

ORCID iD: 0000-0001-7937-2621

10 / 8 Pogodinskaya St., Moscow19121

Russian Federation

V. A. Tutelyan

Federal Research Center for Nutrition, Biotechnology and Food Safety

ORCID iD: 0000-0002-4164-8992

2 / 14 Ustyinsky proezd, Moscow 109240

Russian Federation

N. V. Pyatigorskaya

I.M. Sechenov First Moscow State Medical University, Ministry of Health of Russia (Sechenov University)

ORCID iD: 0000-0003-4901-4625

Bld. 2, 8, Trubetskaya St., Moscow 119991

Russian Federation

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