mRNA of the OY-TES-1 gene in peripheral blood and tumors in colorectal cancer

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Abstract

Background. One of the testicular cancer genes is the OY-TES-1 gene, which encodes a proacrosin-binding protein. This gene is known to be expressed in tumor cells.

Aim. To determinate the detection frequency of OY-TES-1 matrix ribonucleic acid (mRNA) in the peripheral blood and tumor of patients with colorectal cancer (CRC) in comparison with the clinical features of the course of a disease in order to assess the potential monitoring significance of this indicator.

Materials and methods. A method for determining the level of OY-TES-1 mRNA based on reverse transcription polymerase chain reaction (RT-PCR). The detection frequency of mRNA OY-TES-1 gene in blood and tumor samples of 66 patients with CRC, as well as the blood of healthy volunteers, was evaluated.

Results. In the peripheral blood of healthy volunteers, no OY-TES-1 mRNA was detected. In the blood of CRC patients, OY-TES-1 mRNA was detected in 13.6 % of cases. In tumors, it was found in 60,6 % of cases. OY-TES-1 mRNA in the blood and in tumor of patients with CRC was detected statistically significantly more often at stages III and IV than at stages I and II (11,0 and 4,5 times, respectively), and also many times more often in tumors with a low degree of differentiation than with highly differentiated tumors. In patients with CRC, the OY-TES-1 mRNA in tumors with metastases in organs and lymph nodes was detected many times more often than in tumors without any metastases (by 16,4 and 17,4 times respectively).

Conclusion. The data obtained indicate the absence of mRNA OY-TES-1gene in the blood of healthy individuals, the appearance of mRNA OY-TES-1 gene in the blood of CRC patients, and the possibility of using the detection of mRNA OY-TES-1 gene in the blood as an additional informative monitoring marker for colorectal cancer.

About the authors

N. R. Hilal

National Research N. I. Lobachevsky Nizhegorodsky State University; Privolzhsky Research Medical University, Ministry of Health of Russia

Author for correspondence.
Email: nadia.hilal@hotmail.com
ORCID iD: 0000-0001-8367-6118

23 Gagarin ave., Nizhny Novgorod 603022

10 / 1 Minin and Pozharsky sq., Nizhny Novgorod 603000

Russian Federation

D. V. Novikov

National Research N. I. Lobachevsky Nizhegorodsky State University; Academician I. N. Blokhina Nizhny Novgorod Scientific Research Institute of Epidemiology and Microbiology

ORCID iD: 0000-0001-7049-6935

23 Gagarin ave., Nizhny Novgorod 603022

71 Malaya Yamskaya St., Nizhny Novgorod 603022

 

Russian Federation

V. N. Yakimov

National Research N. I. Lobachevsky Nizhegorodsky State University

ORCID iD: 0000-0001-7150-7851

23 Gagarin ave., Nizhny Novgorod 603022

Russian Federation

R. G. Pegov

Nizhny Novgorod Regional Clinical Oncology Center

ORCID iD: 0000-0003-1614-9347

11 / 1 Delovaya St., Nizhny Novgorod 603093

Russian Federation

V. V. Novikov

National Research N. I. Lobachevsky Nizhegorodsky State University; Academician I. N. Blokhina Nizhny Novgorod Scientific Research Institute of Epidemiology and Microbiology

ORCID iD: 0000-0002-2449-7213

23 Gagarin ave., Nizhny Novgorod 603022

71 Malaya Yamskaya St., Nizhny Novgorod 603022

 

Russian Federation

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