Nanoprobes based on fluorescent semiconductor nanocrystals and single-domain antibodies for highly sensitive detection of epidermal growth factor receptor in tumor cells

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Abstract

Background. The development of highly oriented conjugates of quantum dots (QDs) and single-domain antibodies (sdAbs) as innovative fluorescence imaging nanoprobes that specifically recognize tumor biomarkers, in particular, epidermal growth factor receptor (EGFR), is a promising approach to improving immunohistochemical tumor typing.

Aim. The study was aimed at developing fluorescent nanoprobes based on QDs and sdAbs that specifically recognize EGFR, as well as evaluating their functional characteristics (size and optical properties) and functional activity.

Materials and methods. QDs were obtained using high-temperature organometallic synthesis and transferred into the aqueous phase by means of stepwise replacement of ligands on the QD surface. The QDs and sdAbs were conjugated in an oriented manner using a bifunctional cross-linking agent. Detailed characteristics of the resulting conjugates were analyzed by the dynamic light scattering and immunoassay methods. Functional activity was assessed on the model human epidermoid carcinoma cells line A431.

Results. The QD–sdAb conjugates have been standardized in terms of control parameters determining their functional activity, in particular, hydrodynamic diameter and efficiency of binding with target tumor cells. They are characterized by high dispersity, homogeneity, and specific functional activity towards their molecular target.

Conclusion. The results demonstrate the potential use of the designed QD–sdAb conjugates for EGRF detection in immunohistochemical typing of tumor.

About the authors

G. O. Nifontova

University of Reims Champagne-Ardenne

ORCID iD: 0000-0002-6956-5654

51 Cognacq Jay, 51100 Reims, France

France

D. V. Kalenichenko

University of Reims Champagne-Ardenne

ORCID iD: 0000-0002-1394-7768

51 Cognacq Jay, 51100 Reims, France

France

M. A. Baryshnikova

N.N. Blokhin National Medical Research Center of Oncology, Ministry of Health of Russia

ORCID iD: 0000-0002-6688-8423

24 Kashirskoe Shosse, 115522 Moscow, Russia

Russian Federation

Z. A. Sokolova

N.N. Blokhin National Medical Research Center of Oncology, Ministry of Health of Russia

ORCID iD: 0000-0003-4755-5313

24 Kashirskoe Shosse, 115522 Moscow, Russia

Russian Federation

P. S. Samokhvalov

National Research Nuclear University MEPhI (Moscow Engineering Physics Institute)

ORCID iD: 0000-0002-2878-8376

 31 Kashirskoe Shosse, 115409 Moscow, Russia

Russian Federation

A. V. Karaulov

I.M. Sechenov First Moscow State Medical University, Ministry of Health of Russia (Sechenov University)

ORCID iD: 0000-0002-1930-5424

Bld. 2, 8 Trubetskaya, 119992 Moscow, Russia

Russian Federation

A. V. Sukhanova

University of Reims Champagne-Ardenne

ORCID iD: 0000-0003-2796-7898

51 Cognacq Jay, 51100 Reims, France

France

I. R. Nabiev

University of Reims Champagne-Ardenne;
I.M. Sechenov First Moscow State Medical University, Ministry of Health of Russia (Sechenov University)

Author for correspondence.
Email: igor.nabiev@gmail.com
ORCID iD: 0000-0002-8391-040X

Igor Rufailovich Nabiev 

51 Cognacq Jay, 51100 Reims, France

Bld. 2, 8 Trubetskaya, 119992 Moscow, Russia

Russian Federation

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