Comparison of Ridostin Pro and Poly(I:C) as adjuvant for a cancer neoantigen peptide vaccine

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Abstract

Background. The effectiveness of cancer neoantigen peptide vaccines depends on the presence of an adjuvant in their composition. Poly(I:C), a TRL-3 agonist, is used as an adjuvant in mouse models of cancer vaccines, but has limitations for use in humans. Therefore, the search for new effective adjuvants for inclusion in the composition of cancer neoantigen peptide vaccine is relevant. Ridostin Pro is a domestic drug that contains a natural complex of sodium salts of double-chiral and single-chiral ribonucleic acids, is an agonist of TLR-3, an inducer of interferon, its antiviral activity is shown. In this regard, the study of Ridostin Pro as an adjuvant in the composition of neoantigen peptide vaccines is of interest.
Aim. To evaluate the ability of Ridostin Pro and Poly(I:C) adjuvants enhance the specific T-cell response to neoantigen synthetic peptides; to study the antitumor efficacy of a neoantigen peptide vaccine with Ridostin Pro or Poly(I:C) adjuvants.
Materials and methods. Immunogenicity of peptides after vaccination with Ridostin Pro or Poly(I:C) adjuvants evaluated with ELISpot. Antitumor effect of Ridostin Pro or Poly(I:C) adjuvants were evaluated on a mouse model of the B16-F10 tumor by the effect on the tumor growth rate and survival of mice.
Results. Vaccination of mice with Ridostin Pro or Poly(I:C) with neoantigen peptides contributed to the appearance of a specific immune response to peptides that were part of the vaccine. Ridostin Pro, both as part of a vaccine model and when administered without a peptide, inhibits tumor growth and increases the life expectancy of mice with melanoma B16-F10.
Conclusion. Ridostin Pro promotes the formation of a specific immune response to the peptide vaccine, enhances the antitumor effect of the vaccine. These results confirm that Ridostin Pro may prove to be an effective adjuvant for personalized neoantigen peptide vaccines.

About the authors

M. A. Baryshnikova

N.N. Blokhin National Medical Research Center of Oncology, Ministry of Health of Russia

ORCID iD: 0000-0002-6688-8423

24 Kashirskoe Shosse, Moscow 115522

Russian Federation

A. V. Ponomarev

N.N. Blokhin National Medical Research Center of Oncology, Ministry of Health of Russia

Author for correspondence.
Email: kl8546@yandex.ru
ORCID iD: 0000-0001-9517-8183

Alexander Vasilievich Ponomarev

24 Kashirskoe Shosse, Moscow 115522

Russian Federation

A. A. Rudakova

N.N. Blokhin National Medical Research Center of Oncology, Ministry of Health of Russia

ORCID iD: 0000-0001-7266-7689

24 Kashirskoe Shosse, Moscow 115522

Russian Federation

Z. A. Sokolova

N.N. Blokhin National Medical Research Center of Oncology, Ministry of Health of Russia

ORCID iD: 0000-0003-4755-5313

24 Kashirskoe Shosse, Moscow 115522

Russian Federation

N. V. Golubtsova

N.N. Blokhin National Medical Research Center of Oncology, Ministry of Health of Russia

ORCID iD: 0000-0002-8630-1968

24 Kashirskoe Shosse, Moscow 115522

Russian Federation

P. V. Tsarapaev

N.N. Blokhin National Medical Research Center of Oncology, Ministry of Health of Russia

ORCID iD: 0000-0002-1182-1010

24 Kashirskoe Shosse, Moscow 115522

Russian Federation

G. M. Levagina

Institute of Medical Biotechnology of the State Scientific Center of Virology and Biotechnology “Vektor”

ORCID iD: 0000-0002-0394-9698

9 Khimzavodskaya St., Berdsk, Novosibirsk region 630010

Russian Federation

E. D. Danilenko

Institute of Medical Biotechnology of the State Scientific Center of Virology and Biotechnology “Vektor”

ORCID iD: 0000-0001-5026-1602

9 Khimzavodskaya St., Berdsk, Novosibirsk region 630010

Russian Federation

V. S. Kosorukov

N.N. Blokhin National Medical Research Center of Oncology, Ministry of Health of Russia

ORCID iD: 0000-0002-8462-2178

24 Kashirskoe Shosse, Moscow 115522

Russian Federation

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