Cancer-testis genes mRNA presence in tumor lesion and peripheral blood of endometrial cancer patients

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Abstract

Background. Cancer-testicular (CT) genes express in tumor cells in many cancers, including endometrial cancer (ER). CT genes mRNA was detected in the blood of patients with neoplasms, which determines the prospects of using CT gene mRNA as tumor-associated biomarkers. In the peripheral blood of patients with ER, the expression of CT genes has not been studied before.

Objective. Аssessment of occurrence of mRNA of the CT genes MAGEA1–MAGEA6 (MAGEA(1–6)), SSX1, 2, 4, XAGE1, MAGEC1, NY-ESO1 in the tumors and peripheral blood of patients with ER.

Materials and methods. The work used 52 samples of tumor and 54 samples of whole peripheral blood of patients 39–79 years old diagnosed with RE who were treated in clinics in Nizhny Novgorod, as well as the blood of 30 healthy volunteers. In blood and tumors, the frequency of occurrence of mRNA of 12 CT genes was determined using multiplex reverse transcription polymerase chain reaction.

Results. It has been shown that the mRNA of the tested CT genes is detected in ER not only in tumors, but also in the peripheral blood of patients. The most common mRNA is MAGEA(1–6). At the same time, both in tumors and in the peripheral blood of patients, high heterogeneity in the expression of CT genes detected. Moreover, cases of MAGEA(1–6), SSX1, 2, 4, XAGE1, NY-ESO1, MAGEC1 mRNA detection in tumors were revealed in the absence of their detection in the peripheral blood and conversely. The total frequency of MAGEA(1–6), SSX1, 2, 4, XAGE1, NY-ESO1, MAGEC1 mRNA occurrence in tumors was 75.0 %, and in peripheral blood – 44.6 %. As in tumors, most blood samples showed expression of only one CT gene. Only in some cases the expression of two or three genes was recorded. Note that in all blood samples of healthy volunteers mRNA CT genes was not detected. Additionally, mixed form of macroscopic growth (both exophytic and endophytic), growth along the uterine fundus and walls, as well as low degree of differentiation were followed by a higher mRNA CT genes occurrence in the blood and tumors.

Conclusion. In ER mRNA CT genes occur in the blood and tumors of patients. A more severe course of ER is associated with an increase in the incidence of mRNA CT genes.

About the authors

A. V. Kalugin

N. I. Lobachevsky National Research State University of Nizhny Novgorod

ORCID iD: 0000-0002-5484-5645

Alexander V. Kalugin

23 Gagarin Ave., Nizhny Novgorod 603950

Russian Federation

D. V. Novikov

N. I. Lobachevsky National Research State University of Nizhny Novgorod; Academician N. I. Blokhina Nizhny Novgorod Scientific Research Institute of Epidemiology and Microbiology

ORCID iD: 0000-0001-7049-6935

Dmitriy V. Novikov

23 Gagarin Ave., Nizhny Novgorod 603950
71 Malaya Yamskaya St., Nizhny Novgorod 603950

Russian Federation

M. E. Mamaeva

Medical Center of Privolzhsky District, Federal Medical Biological Agency

ORCID iD: 0000-0001-5961-7986

Marina E. Mamaeva

2 Nizhnevolzhskaya Embankment, Nizhny Novgorod 603001

Russian Federation

A. V. Alyasova

Privolzhsky Research Medical University, Ministry of Health of Russia

ORCID iD: 0000-0003-2621-0359

10 / 1 Minin and Pozharsky Sq., Nizhny Novgorod 603950

Аnna V. Alyasova

Russian Federation

Kh. M. Arioua

N. I. Lobachevsky National Research State University of Nizhny Novgorod

ORCID iD: 0000-0003-1179-2552

Khalil M. Arioua

23 Gagarin Ave., Nizhny Novgorod 603950

Russian Federation

V. V. Novikov

N. I. Lobachevsky National Research State University of Nizhny Novgorod; Academician N. I. Blokhina Nizhny Novgorod Scientific Research Institute of Epidemiology and Microbiology

Author for correspondence.
Email: mbre@mail.ru
ORCID iD: 0000-0002-2449-7213

Viktor Vladimirovich Novikov

23 Gagarin Ave., Nizhny Novgorod 603950
71 Malaya Yamskaya St., Nizhny Novgorod 603950

Russian Federation

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