Email this article FEASIBILITY STUDY OF MAGNETIC RESONANCE IMAGINING APPLICATION IN EXPERIMENTAL RADIOLOGY FOR INTRAVITAL VERIFICATION OF LUNGS METASTASES IN MICE
- Authors: Smirnova A.V.1,2, Varaksa P.O.1, Finogenova Y.A.1, Lagodzinskaya Y.A.1, Lipengolts A.A.1,3,4, Abakumov M.A.5,6, Grigorieva E.Y.1
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Affiliations:
- N.N. Blokhin National Medical Research Center of Oncology, Ministry of Health of Russia
- A.S. Loginov Moscow Clinical Scientific Center, Moscow Healthcare Department
- A.I. Burnazyan Federal Medical Biophysical Center, FMBA of Russia
- National Research Nuclear University MEPhI (Moscow Engineering Physics Institute)
- N.I. Pirogov Russian National Research Medical University, Ministry of Health of Russia
- National University of Science and Technology “MISIS”
- Issue: Vol 20, No 2 (2021)
- Pages: 69-75
- Section: ORIGINAL REPORTS
- Published: 14.07.2021
- URL: https://bioterapevt.abvpress.ru/jour/article/view/1260
- DOI: https://doi.org/10.17650/1726-9784-2021-20-2-69-75
- ID: 1260
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Abstract
Background. For preclinical studies of radiopharmaceuticals there is a high need for development new methods of in vivo metastasis diagnosis in mice after tumor cells transplantation. The study was carried out to assess feasibility of lung metastasis diagnosis with magnetic resonance imaging (MRI) visualization in mice C57Bl6.
The aim of this study was to assess feasibility of MRI for verification of distant metastases of the solid B16-F10 melanoma with validation by anatomic dissection.
Methods. Metastatic lesions were caused by injection of B16-F10 murine melanoma cells into cavum medullare of the tibia. Imaging studies were performed on the 21th day after transplantation using 7T magnetic resonance tomograph, coronal and axial images were acquired. Validation of metastasis was made by anatomic dissection and histological examination.
Results. MRI method enables visualization of lung nodules with diameter at least 0.8 mm, because smaller nodules cannot be distinguished from heartbeat artifacts. Histological examination revealed that macroscopic anatomic dissection can precisely detect subpleural lung nodules.
Conclusion. This study demonstrated feasibility of in vivo lung metastasis verification with MRI method in mice: metastases comparable in diameter to the size of large bronchi can be detected by MRI as well as by ex vivo dissection. For centrally located lung metastasis MRI method is preferable because macroscopic dissection enables to visualize only nodules which are located subpleurally.
About the authors
A. V. Smirnova
N.N. Blokhin National Medical Research Center of Oncology, Ministry of Health of Russia; A.S. Loginov Moscow Clinical Scientific Center, Moscow Healthcare Department
Author for correspondence.
Email: smirn-ova@mail.ru
ORCID iD: 0000-0003-0386-9732
Anna Vyacheslavna Smirnova
24 Kashirskoe Shosse, Moscow 115478
86 Entuziastov Shosse, Moscow 111123
Russian FederationP. O. Varaksa
N.N. Blokhin National Medical Research Center of Oncology, Ministry of Health of Russia
ORCID iD: 0000-0003-0106-8384
24 Kashirskoe Shosse, Moscow 115478
Russian FederationYu. A. Finogenova
N.N. Blokhin National Medical Research Center of Oncology, Ministry of Health of Russia
ORCID iD: 0000-0002-5144-1039
24 Kashirskoe Shosse, Moscow 115478
Russian FederationYu. A. Lagodzinskaya
N.N. Blokhin National Medical Research Center of Oncology, Ministry of Health of Russia
ORCID iD: 0000-0001-6260-0763
24 Kashirskoe Shosse, Moscow 115478
Russian FederationA. A. Lipengolts
N.N. Blokhin National Medical Research Center of Oncology, Ministry of Health of Russia; A.I. Burnazyan Federal Medical Biophysical Center, FMBA of Russia; National Research Nuclear University MEPhI (Moscow Engineering Physics Institute)
ORCID iD: 0000-0002-5631-9016
24 Kashirskoe Shosse, Moscow 115478
46 Zhivopisnaya St., Moscow 123098
31 Kashirskoe Shosse, Moscow 115409
Russian FederationM. A. Abakumov
N.I. Pirogov Russian National Research Medical University, Ministry of Health of Russia; National University of Science and Technology “MISIS”
ORCID iD: 0000-0003-2622-9201
1 Ostrovityanova St., Moscow 117997
4 Leninskiy Ave., Moscow 119049
Russian FederationE. Yu. Grigorieva
N.N. Blokhin National Medical Research Center of Oncology, Ministry of Health of Russia
ORCID iD: 0000-0001-7726-7991
24 Kashirskoe Shosse, Moscow 115478
Russian FederationReferences
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