Email this article SELECTION OF A CRYOPROTECTOR FOR PRODUCTION A LYOPHILIZED LIPOSOMAL DOSAGE FORM OF THE INDOLOCARBAZOLE DERIVATIVE LHS‑1269
- Authors: Dmitrieva M.V.1, Lugen B.2, Polozkova A.Р.1, Orlova О.L.1, Krasnyuk I.I.2, Krasnyuk(jr.) I.I.2
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Affiliations:
- N.N. Blokhin National Medical Research Center of Oncology of the Ministry of Health of the Russian Federation
- I.M. Sechenov First Moscow State Medical University, Ministry of Health of Russia
- Issue: Vol 20, No 1 (2021)
- Pages: 74-79
- Section: ORIGINAL REPORTS
- Published: 08.04.2021
- URL: https://bioterapevt.abvpress.ru/jour/article/view/1247
- DOI: https://doi.org/10.17650/1726-9784-2021-20-1-74-79
- ID: 1247
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Abstract
Introduction. One of the key tasks in freeze‑drying of drug is to choose the optimal cryoprotector that provides a high‑quality lyophilized product.
The aim of research. Selection of cryoprotectant and its concentration for the preparation of lyophilizate of the liposomal dosage form of the indolocarbazole derivative LHS‑1269.
Materials and methods. Substance LHS‑1269 ≥99 % (N. N. Blokhin National Medical Research Center of Onco logy), egg phosphatidylcholine Е РС S (Lipoid, Germany), cholesterol ≥99 % (Sigma‑Aldrich, Japan), polyethylene glycol‑2000‑distearoyl phosphatidylethanolamine (Lipoid, Germany), mannose‑d (+) 99 % (Kaden, Germany), sucrose (Himmed, Russia), trehalose dihydrate (Himmed, Russia). To obtain LHS‑1269 liposomes, the Bangham method was used in modification for hydrophobic substances with subsequent extrusion of the dispersion of multilayer phos‑ pholipid vesicles. The prepared liposomal dispersion was dosed into vials of 6 ml and lyophilized in the freeze‑dryng chamber using the «step‑by‑step» freezing mode. LHS‑1269 liposomes were analyzed before and after freeze‑drying using laser scattering spectroscopy and determination of the electrophoretic mobility of particles.
Results. To prevent the destruction of LHS‑1269 liposomes during lyophilization, substances from the carbohy‑ drates class – mannose, sucrose and trehalose – were studied in two concentrations. In the course of compara‑ tive evaluation of the obtained lyophilizates in terms of quality, appearance, rehydration, size and zeta potential of liposomes before and after sublimation, it was found that sucrose introduced into the liposomal dosage form LHS‑1269 in the molar ratio sucrose / egg phosphatidylcholine 5:1 has optimal cryoprotective properties.
Conclusion. As a result of the study, the optimal cryoprotector and its concentration were selected, which ensure the production of high‑quality lyophilizate of the liposomal composition of the indolocarbazole derivative LHS‑1269.
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About the authors
M. V. Dmitrieva
N.N. Blokhin National Medical Research Center of Oncology of the Ministry of Health of the Russian Federation
Author for correspondence.
Email: dmitrieva.m@ronc.ru
ORCID iD: 0000-0001-6740-5692
Maria Vyacheslavovna Dmitrieva
24 Kashirskoe Shosse, Moscow 115478,
Russian FederationBu Lugen
I.M. Sechenov First Moscow State Medical University, Ministry of Health of Russia
ORCID iD: 0000-0002-7740-5562
Build 2, 8 Trubetskaya St., Moscow 119991
Russian FederationA. Р. Polozkova
N.N. Blokhin National Medical Research Center of Oncology of the Ministry of Health of the Russian Federation
24 Kashirskoe Shosse, Moscow 115478,
Russian FederationО. L. Orlova
N.N. Blokhin National Medical Research Center of Oncology of the Ministry of Health of the Russian Federation
ORCID iD: 0000-0002-4558-0083
24 Kashirskoe Shosse, Moscow 115478,
Russian FederationI. I. Krasnyuk
I.M. Sechenov First Moscow State Medical University, Ministry of Health of Russia
ORCID iD: 0000-0003-4382-7377
Build 2, 8 Trubetskaya St., Moscow 119991
Russian FederationI. I. Krasnyuk(jr.)
I.M. Sechenov First Moscow State Medical University, Ministry of Health of Russia
ORCID iD: 0000-0001-8557-8829
Build 2, 8 Trubetskaya St., Moscow 119991
Russian FederationReferences
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