Email this article Lymphocytes blast-transformation reaction: modification for allergological practice
- Authors: Manina I.V.1, Sergeev V.Y.1, Golubtsova N.V.2, Sergeev A.Y.3
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Affiliations:
- Institute of Allergology and Clinical Immunology
- N.N. Blokhin National Medical Research Center of Oncology, Ministry of Health of Russia
- I.M. Sechenov First Moscow State Medical University, Ministry of Health of Russia (Secheniv University)
- Issue: Vol 17, No 2 (2018)
- Pages: 88-92
- Section: ORIGINAL REPORTS
- Published: 24.11.2018
- URL: https://bioterapevt.abvpress.ru/jour/article/view/1092
- DOI: https://doi.org/10.17650/1726-9784-2018-17-2-88-92
- ID: 1092
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Abstract
Introduction. Lymphocytes blast-transformation reaction (RBLT) is necessary for patient’s inspection with immunologic infringements. It is applied in the different fields of medicine to identification of a sensitization to antigens (allergens).
Research objective – to modify and automate RBTL for application in routine laboratory practice.
Materials and methods. RBTL, antigens (allergens). Software: Windows 7; Intel Pentium G4500 CPU. Digital CMOS video camera, Mikmed-6 microscope.
Results. We analyzed difficulties when using RBTL in laboratory allergological practice. We created program files for automation of RBTL.
Conclusion. We adapted and automated RBTL for laboratory diagnostics using.
About the authors
I. V. Manina
Institute of Allergology and Clinical Immunology
Author for correspondence.
Email: Irina.v.manina@gmail.com
Bldg 1, 20 Malaya Bronnaya St., Moscow 123104
Russian FederationV. Yu. Sergeev
Institute of Allergology and Clinical Immunology
Bldg 1, 20 Malaya Bronnaya St., Moscow 123104
Russian FederationN. V. Golubtsova
N.N. Blokhin National Medical Research Center of Oncology, Ministry of Health of Russia
24 Kashirskoe Shosse, Moscow 115478
Russian FederationA. Yu. Sergeev
I.M. Sechenov First Moscow State Medical University, Ministry of Health of Russia (Secheniv University)
Bldg 4, 2 Bol’shaya Pirogovskaya St., Moscow 119991
Russian FederationReferences
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